Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro
Regulação peptídica das funções de fibroblastos cutâneos durante o envelhecimento in vitro
Lin'kova NS, Drobintseva AO, Orlova OA, Kuznetsova EP, Polyakova VO, Kvetnoy IM, Khavinson VKh
Bull Exp Biol Med
Summary
This in vitro study evaluated the effects of Vesugen (tripeptide KED) on human skin fibroblasts at different stages of cellular aging (early versus late passages). Researchers analyzed the expression of proliferation, regeneration, and extracellular matrix degradation markers by immunocytochemistry.
The results demonstrated that in vitro aging of fibroblasts led to an increase in MMP-9 synthesis (matrix metalloproteinase 9), an enzyme that degrades collagen and other extracellular matrix components. Treatment with Vesugen significantly inhibited this MMP-9 increase, protecting extracellular matrix integrity.
- Ki-67 expression (proliferation marker) was increased by Vesugen in aged fibroblasts
- The CD98hc marker (associated with regeneration and amino acid transport) also had its expression elevated
- The combined effect suggests restoration of proliferative and regenerative capacity
- Treated fibroblasts exhibited morphology more consistent with young cells
These findings are relevant for understanding skin aging, in which the increase of MMPs and reduction of fibroblast regenerative capacity are central events. Vesugen demonstrated potential to simultaneously counterbalance these two deleterious processes, suggesting applications in the field of regenerative dermatology and anti-aging cosmeceuticals.
Related Peptide
Vesugen
Peptídeo vascular
Vascular bioregulatory tripeptide developed by the Khavinson group. Regulates gene expression in vascular endothelium and supports the integrity of blood vessel walls. Used in short cycles for vascular system protection and support.