Specialized Research

NAD+

Also known as: Nicotinamide Adenine Dinucleotide

Molecular Identifiers

Molecular Formula

C21H27N7O14P2

PubChem CID

5892

Molecular Weight

663.43 Da

Overview

Essential coenzyme for cellular energy metabolism. Declines with age. Supplementation aims to restore cellular levels to support DNA repair and mitochondrial function.

The main clinical interest around NAD+ is to restore cellular levels that decline with age, sustaining the activity of sirtuins (SIRT1-7), PARP (DNA repair), the mitochondrial electron transport chain, and circadian clock regulation. Sought effects include energy, recovery, cognitive support, and longevity context. Clinical evidence of functional impact in humans remains limited and mixed.

Technically not a peptide but a dinucleotide coenzyme, often categorized alongside peptides in the longevity market. There is no FDA/EMA/ANVISA regulatory approval for injectable NAD+ as therapy; it is widely administered in wellness clinics via intravenous or subcutaneous infusion as off-label use, with protocols of 50-250 mg, 2-3 times per week. It can cause flushing, nausea, and chest discomfort with rapid infusions — starting with a low dose is standard practice.

Small molecule — dinucleotide coenzyme (nicotinamide adenine dinucleotide), not a peptide

Half-life

~30 minutes (IV)

Administration Route

Intravenous or subcutaneous

Category

Specialized Research

Mechanism of Action

  • Essential cofactor for sirtuins (SIRT1-7)
  • Substrate for PARP (DNA repair)
  • Support for the mitochondrial electron transport chain
  • Circadian clock regulation

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose 50-250 mg per injection
Frequency 2-3 times per week
Timing Preferably morning
Duration Continuous as tolerated

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Flushing
  • Nausea
  • Cramps
  • Chest discomfort (rapid infusion)
  • Headache

Product Properties

Purity >98%
Appearance White crystalline powder
Solubility Freely soluble in water
Source Chemical synthesis or enzymatic production
Storage Store at -20°C desiccated for up to 2 years. At 2-8°C desiccated for up to 6 months. Reconstituted: use immediately or store at -20°C. Hygroscopic — protect from moisture.

Presentations & Preparation

Vials of NAD+ found in the research market:

20 mg100 mg500 mg750 mg1000 mg

Reconstitution

  • Diluent: Bacteriostatic water
  • Volume: According to concentration
  • Inject the diluent slowly
  • Gently swirl until fully dissolved
  • May cause flushing — start with a low dose

Storage

  • Lyophilized: -20°C or refrigerated 2-8°C
  • Reconstituted: Refrigerated 2-8°C (up to 14 days)
  • Limited stability after reconstitution
  • Protect from direct light
Reconstitution Calculator

Scientific Studies

Published studies on NAD+.

2015 · Cell Metab

NAD+ metabolism and the control of energy homeostasis: A balancing act between mitochondria and the nucleus

Cantó C, Menzies KJ, Auwerx J
2015 · Science

NAD+ in aging, metabolism, and neurodegeneration

Verdin E

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