Specialized Research

NAD+

Also known as: Nicotinamide Adenine Dinucleotide

Molecular Identifiers

Molecular Formula

C21H27N7O14P2

PubChem CID

5892

Overview

Essential coenzyme for cellular energy metabolism. Declines with age. Supplementation aims to restore cellular levels to support DNA repair and mitochondrial function.

Small molecule — dinucleotide coenzyme (nicotinamide adenine dinucleotide), not a peptide

Half-life

~30 minutes (IV)

Administration Route

Intravenous or subcutaneous

Category

Specialized Research

Mechanism of Action

  • Essential cofactor for sirtuins (SIRT1-7)
  • Substrate for PARP (DNA repair)
  • Support for the mitochondrial electron transport chain
  • Circadian clock regulation

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose 50-250 mg per injection
Frequency 2-3 times per week
Timing Preferably morning
Duration Continuous as tolerated

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Flushing
  • Nausea
  • Cramps
  • Chest discomfort (rapid infusion)
  • Headache

Presentations & Preparation

Vials of NAD+ found in the research market:

20 mg100 mg500 mg750 mg1000 mg

Reconstitution

  • Diluent: Bacteriostatic water
  • Volume: According to concentration
  • Inject the diluent slowly
  • Gently swirl until fully dissolved
  • May cause flushing — start with a low dose

Storage

  • Lyophilized: -20°C or refrigerated 2-8°C
  • Reconstituted: Refrigerated 2-8°C (up to 14 days)
  • Limited stability after reconstitution
  • Protect from direct light
Reconstitution Calculator

Scientific Studies

Published studies on NAD+.

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