Cosmetic & Skin

GHK Basic

Also known as: GHK, Gly-His-Lys

Molecular Identifiers

Molecular Formula

C14H24N6O4

CAS Number

49557-75-7

PubChem CID

73587

Molecular Weight

340.38 Da

Overview

Copper-binding tripeptide in its base form (without complexed copper). Naturally present in human plasma, colostrum, and saliva. Promotes collagen synthesis, wound healing, and tissue remodeling. Precursor to GHK-Cu.

Clinical interest in base GHK centers on type I and III collagen synthesis, wound healing, extracellular matrix remodeling, and anti-inflammatory modulation. It is the version without pre-complexed copper, capable of binding the ion in vivo. Endogenous plasma levels decline with age — from around 200 ng/ml in youth to roughly 80 ng/ml around age 60 — supporting interest in supplemental dermatological use and tissue repair.

GHK has no approval as a drug, but it is widely used in topical cosmetics and as off-label injectable supplementation. It is typically dispensed by compounding pharmacies in topical formulations (serums, creams) and, less frequently, in subcutaneous form, in 4–12 week cycles. There is no specific athletic indication or WADA ban.

Within the GHK peptide family, base GHK differs from GHK-Cu by not having pre-complexed copper: the copper-bound version is the gold standard in regenerative and copper-cosmetic formulations, with more robust and documented biological activity. Pal-GHK (Palmitoyl Tripeptide-1) attaches a lipophilic tail to GHK for strictly topical use, while AHK-Cu, an alanine analogue, is geared toward hair follicle stimulation.

Sequence (1 letter): GHK
Extended notation: Gly-His-Lys

Half-life

~2-4 hours

Administration Route

Topical or subcutaneous

Category

Cosmetic & Skin

Mechanism of Action

  • Stimulation of type I and III collagen synthesis
  • Promotion of wound healing and tissue repair
  • Anti-inflammatory activity via cytokine modulation
  • Extracellular matrix remodeling
  • Attraction of mesenchymal stem cells to the repair site

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose Topical or 200-600 mcg subcutaneous
Frequency 1-2 times daily (topical), every other day (subcutaneous)
Timing Preferably at night
Duration 4-12 weeks

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Mild skin irritation
  • Local redness

Product Properties

Purity >98%
Appearance White lyophilized powder
Solubility Soluble in water and bacteriostatic water
Source Solid-phase peptide synthesis (SPPS)
Storage Lyophilized: -20°C for up to 2 years, 2-8°C for up to 6 months. Reconstituted: 2-8°C for up to 4 weeks. Protect from light and moisture. Avoid repeated freeze-thaw cycles.

Presentations & Preparation

Vials of GHK Basic found in the research market:

10 mg20 mg50 mg100 mg

Reconstitution

  • Diluent: Bacteriostatic water
  • Volume: 2 ml per vial
  • Slowly inject the diluent along the vial wall
  • Gently swirl until fully dissolved
  • Never shake

Storage

  • Lyophilized: Refrigerated 2-8°C
  • Reconstituted: Refrigerated 2-8°C (up to 30 days)
  • Protect from direct light
  • Do not freeze after reconstitution
Reconstitution Calculator

Scientific Studies

Published studies on GHK Basic.

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