Cognitive & Neuroprotective

Humanin

Also known as: HN, HNG (S14G-Humanin)

Molecular Identifiers

Molecular Formula

C119H204N34O32S2

PubChem CID

16131438

Molecular Weight

2687 Da

Overview

Mitochondrial genome-derived peptide with 24 amino acids. Has potent neuroprotective and anti-apoptotic effects. Acts on insulin sensitization and protection against neurodegenerative diseases and metabolic stress.

Sequence (1 letter): MAPRGFSCLLLLTSEIDLPVKRRA
Extended notation: Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala

Half-life

~30 minutes

Administration Route

Subcutaneous

Category

Cognitive & Neuroprotective

Mechanism of Action

  • Neuroprotection against beta-amyloid toxicity and oxidative stress
  • Anti-apoptotic effect via interaction with Bax and IGFBP-3
  • Insulin sensitization and metabolic improvement
  • Mitochondrial protection and maintenance of bioenergetic function
  • Reduction of systemic inflammation and endoplasmic reticulum stress

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose 1-5 mg per day subcutaneous (research)
Frequency Once daily
Timing Morning or per research protocol
Duration 4-12 weeks

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Injection site pain (mild)
  • Headache (rare)

Presentations & Preparation

Vials of Humanin found in the research market:

5 mg10 mg15 mg20 mg

Reconstitution

  • Diluent: Bacteriostatic water
  • Volume: 2 ml per vial
  • Inject the diluent slowly against the vial wall
  • Gently swirl until fully dissolved
  • Never shake

Storage

  • Lyophilized: -20°C or refrigerated 2-8°C
  • Reconstituted: Refrigerated 2-8°C (up to 14 days)
  • Protect from direct light
  • Do not freeze after reconstitution
  • Sensitive peptide — store at stable temperature
Reconstitution Calculator

Scientific Studies

Published studies on Humanin.

2019 · Front Aging Neurosci

Humanin, a Mitochondrial-Derived Peptide Released by Astrocytes, Prevents Synapse Loss in Hippocampal Neurons

Zárate SC, Traetta ME, Codagnone MG, Seilicovich A, Reinés AG
2020 · Aging (Albany NY)

The Mitochondrial Derived Peptide Humanin Is a Regulator of Lifespan and Healthspan

Yen K, Mehta HH, Kim SJ, Lue YH, Hoang J, Guerrero N, Port J, Bi Q, et al.

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