Healing & Recovery

MGF

Also known as: Mechano Growth Factor, PEG-MGF, IGF-1Ec

Molecular Identifiers

Molecular Formula

F3Mg-

PubChem CID

447728

Molecular Weight

~2.867 Da

Overview

Mechano Growth Factor, a splicing variant of IGF-1 (IGF-1Ec isoform) expressed in muscle tissues after mechanical stimulus. Activates muscle satellite cells, promoting proliferation and fusion for repair and hypertrophy. The PEGylated form (PEG-MGF) has an extended half-life of ~48-72 hours.

Sequence (1 letter): YQPPSTNKNTKSQRRKGSTFEERK
Extended notation: Tyr-Gln-Pro-Pro-Ser-Thr-Asn-Lys-Asn-Thr-Lys-Ser-Gln-Arg-Arg-Lys-Gly-Ser-Thr-Phe-Glu-Glu-Arg-Lys

C-terminal domain of 24 amino acids from the IGF-1Ec isoform. The PEG-MGF form includes PEGylation to extend the half-life.

Half-life

~5-7 minutes (native MGF) / ~48-72 hours (PEG-MGF)

Administration Route

Intramuscular or subcutaneous

Category

Healing & Recovery

Mechanism of Action

  • Activation of muscle satellite (progenitor) cells, promoting proliferation and fusion
  • Anti-apoptotic effect via caspase-3 inhibition, protecting muscle and cardiac cells
  • Autonomous neuroprotective effect of the C-terminal peptide in cerebral ischemia models
  • Promotion of angiogenesis and tissue repair at sites of mechanical injury
  • Modulation of inflammatory cytokines and macrophage resolution in muscle injury

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose 200-400 mcg per injection
Frequency Once daily (MGF) or 2-3 times per week (PEG-MGF)
Timing Immediately after training (30-60 minutes)
Duration 4-6 weeks

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Pain and redness at injection site
  • Mild hypoglycemia at high doses
  • Localized swelling from water retention
  • Joint and muscle pain
  • Fatigue and headache (rare)

Presentations & Preparation

Vials of MGF found in the research market:

2 mg

Reconstitution

  • Diluent: Bacteriostatic water
  • Volume: 1-2 ml per 2 mg vial
  • Inject slowly against the vial wall
  • Gently swirl until fully dissolved
  • Never shake vigorously

Storage

  • Lyophilized: Frozen -20°C (up to 24 months)
  • Reconstituted: Refrigerated 2-8°C (up to 7-10 days for native MGF, up to 30 days for PEG-MGF)
  • Avoid freeze/thaw cycles
  • Protect from direct light
  • PEG-MGF has slightly better stability than native MGF
Reconstitution Calculator

Scientific Studies

Published studies on MGF.

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