Cosmetic & Skin

Tripeptide-29

Also known as: Collagen Tripeptide, GHK Mimic

Molecular Identifiers

Molecular Formula

C12H19N3O5

CAS Number

2239-67-0

PubChem CID

11778669

Molecular Weight

285.30 Da

Overview

Synthetic tripeptide that mimics collagen fragments, stimulating type I collagen production in the dermis. Sequence based on repetitive motifs of the collagen alpha chain.

Tripeptide-29 is developed as a mimetic of collagen fragments, signaling dermal fibroblasts to stimulate type I collagen synthesis. The expected effect is improved skin elasticity and reinforcement of the dermal barrier, with continuous use. It is often combined with other peptides such as Matrixyl and Argireline in multi-peptide formulations to enhance the anti-aging effect.

Today it is used almost exclusively as a cosmetic ingredient, at typical concentrations of 50 to 200 ppm in serums, creams, and professional formulations sold over the counter. It has no approval as a medicine and is not given by injection. The exact structure may vary between manufacturers, which makes direct product-to-product comparison difficult.

Among cosmetic peptides, Tripeptide-29 is mechanistically close to Matrixyl: both signal fibroblasts to increase collagen synthesis, with no neuromuscular action. It differs fundamentally from Argireline, SNAP-8, and Acetyl Octapeptide-1 (which act on the SNARE pathway) and from Decapeptide-12 (depigmentation). As a simple and inexpensive tripeptide, it is often used as a complementary ingredient in formulations that already contain Matrixyl or SNARE-group peptides.

Sequence (1 letter): GPO

Collagen motif-based tripeptide. Structure varies between manufacturers.

Half-life

Variable (topical use)

Administration Route

Topical

Category

Cosmetic & Skin

Mechanism of Action

  • Stimulation of type I collagen synthesis
  • Signaling to dermal fibroblasts
  • Improvement of skin elasticity
  • Strengthening of the dermal barrier

Dosage Protocol

Data compiled from the literature. This does not constitute medical advice.

Parameter Value
Dose Topical application
Frequency 1-2 times daily
Timing Morning and/or evening
Duration Continuous use

Reported Side Effects

Adverse effects described in the literature. Severity and frequency vary between individuals.

  • Mild skin irritation (very rare)

Product Properties

Purity >98%
Appearance White lyophilized powder
Solubility Soluble in water and bacteriostatic water
Source Solid-phase peptide synthesis (SPPS)
Storage Lyophilized: -20°C for up to 2 years, 2-8°C for up to 6 months. Reconstituted: 2-8°C for up to 4 weeks. Protect from light and moisture. Avoid repeated freeze-thaw cycles.

Presentations & Preparation

Vials of Tripeptide-29 found in the research market:

100 mg200 mg

Reconstitution

  • Diluent: N/A (topical use)
  • Volume: N/A
  • Supplied as powder or incorporated into formulations
  • Typical concentration 50-200 ppm

Storage

  • Lyophilized: Room temperature
  • Reconstituted: N/A
  • Protect from light
  • Store in a cool, dry place
Reconstitution Calculator

Scientific Studies

Published studies on Tripeptide-29.

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